Taq DNA Polymerase

Source

E.coli

Description

Taq DNA polymerase is a thermostable DNA polymerase I enzyme named after the thermophilic eubacterial microorganism Thermus aquaticus, from which it was originally isolated. It is approximately 95 KDa, replicates DNA at 74°C, and exhibits a half-life of 40 minutes at 95°C. The enzyme catalyses the polymerization of nucleotides into duplex DNA in the presence of magnesium and possesses exonuclease activity. It is highly recommended for use in PCR.

Species

Formulation buffer

20mM Tris, 100Mm KCl, 0.1mM EDTA, 1mM DTT, 0.5% NP40, 0.5% Tween20, 50% Glycerol, pH: 8.0±0.2

10X Reaction Buffer

100mM Tris (pH: 9.0±0.2), 500mM KCl, 1% Triton X100, 15mM MgCl2

Form

Liquid

Expiry

1 year

Storage temperature

-20°C