Reverse Transcriptase

Source

E.coli

Description

HIV-1 reverse transcriptase is an RNA-dependent DNA polymerase derived from HIV-1 (AIDS virus), subtype B origin that uses reverse transcription as a step in the process of replication. At YBL, the protein is over-expressed as a recombinant protein in E.coli. It is composed of two sub-units with molecular weight of 66 KDa and 51 KDa, same as enzyme purified from HIV virus particles.

Species

HIV1

Formulation buffer

50mM Tris, 150Mm NaCl, 5mM MgCl2, 1mM DTT, 0.1% Triton X100, 50% Glycerol, pH: 8.3±0.2

Form

Liquid

Expiry

1 year

Storage temperature

-20°C