iPSC-derived Cardiomyocytes – YBLiCardio
Datasheet
Mature Cardiac Marker Expression in YBLiCardio Cells and Post Freeze-Thaw Enhancements
YBLiCardio cells displayed mature cardiac characteristics, including the expression of cardiac troponin. Furthermore, after undergoing freeze-thaw cycles, these cells exhibited increased levels of Troponin, MLV2a, and MLC2v, likely due to extended post-freeze-thaw culture.
Confocal Images of YBiCardio Expressing α –Actinin
Rt-qPCR Analysis of Mature Cardiac Markers in YBLiCardio Cells
Rt-qPCR analysis to investigate the expression of mature cardiac markers in YBLiCardio cells.
Action Potential Recording and Ionic Currents Assessment – Acute Toxicity
Maintaining consistent myocardial contractility (inotropy) is crucial for proper cardiac function, as deviations can result in adverse cardiac events, including contractile dysfunction, arrhythmias, and heart failure, often complicating the use of innovative treatments. To address this challenge, human-induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) have emerged as a valuable tool for predicting cardiotoxicity in a clinical context. We conducted a study to investigate the connection between contraction parameters and beating rates in YBLiCardio. Our approach involved direct measurement of contraction force and a comparison of the effects of ion channel drugs, including Nifedipine, Lidocaine, E-4031, and Isoproterenol, on contraction parameters. YBLiCardio offers human-specific insights and a vital tool in drug development and toxicology for evaluating chronic cardiotoxicity.
FLEXcyte 96 platform was utilized to monitor the drug response and essential parameters such as amplitude, duration, rate, Area Under Curve (AUC), and the slopes of the contraction and relaxation phases were calculated to determine the cardiac contractility.
A) Nifedipine, a dihydropyridine derivative, was used for the assessment of calcium handling of the cells at concentrations ranging from 1 nM to 1 μM. The compound showed the expected negative inotropic responses by blocking L-type Ca2+ channels of the cardiomyocytes. At 1 µM, the cells ceased beating.
B) Lidocaine, a sodium channel modulator, had a concentration-dependent negative inotropic effect on YBLiCardio cells.
C) E-4031, a potassium channel antagonist(hERG channels) treatment revealed the occurrence of arrhythmic events at concentrations of 100 nM and higher, underlining the potential proarrhythmic effects associated with E-4031. This included early after contractions (EAC) events as well as fibrillations intermittently with phases of regular beating.
D) The beta-adrenergic stimulator isoproterenol induced significant positive inotropic responses of YBLiCardio cells, indicating their proper mature state.
Action Potential Recording and Ionic Currents Assessment – Chronic Toxicity
A) Doxorubicin had a time- and concentration-dependent negative inotropic effect on YBLiCardio cells.
B) The tyrosine kinase inhibitor Erlotinib a low cardiac toxic compound had no effect on the contractile properties of YBLiCardio cells.
C) Pentamidine a hERG- trafficking disrupter, showed the expected time- and concentration-dependent positive effects.
D) The tyrosine kinase inhibitor sunitinib demonstrated a concentration-dependent negative effect on the contractile properties of YBLiCardio cells.
Comparative RNA-seq Analysis of YBLiCardio Gene Expression Over Time with Human Heart RNA
RNA sequencing (RNA-seq) study, comparing the gene expression profiles of YBLiCardio cells at different time points (Day 0, Day 6, Day 15, and Day 30) with those of adult human heart RNA. This analysis allowed us to explore changes in gene expression over time, providing insights into the maturation and development of YBLiCardio cells in comparison to adult human heart tissue."
Stable Gene Expression Data for cardiac genes demonstrate a stable genomic expression profile YBLiCardio that trends well with that of adult human cardiac tissue.
