Human Peripheral Blood Mononuclear Cells (PBMCs)

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Peripheral Blood Mononuclear Cells are isolated from whole blood, leukapheresis, or whole blood samples using density gradient separation or red blood cell lysis and cryopreserved. All peripheral blood samples are obtained from donors diagnosed with cancer, autoimmune or lifestyle diseases with Institutional Ethics Committee (IEC)-approved consent forms/waivers and protocols. Sodium heparin/EDTA/Sodium Citrate is added during collection to the vacuutainer tube as an anticoagulant during peripheral blood collection. PBMCs form the starting source for isolation of several types of immune cells, HLA analysis as well as immune function

Note- Donors are screened for HIV ½, HBV and HCV viral markers before blood collection

The PBMCs isolated from the Buffy coat are verified for their viability and purity (Refer Fig 1 below).

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