This conference poster presents a framework for developing matched monoclonal antibody (mAb) pairs against thyroglobulin (TG), an important biomarker used in thyroid cancer diagnostics. Due to its large size and complex structure, identifying effective antibody pairs for TG detection can be challenging during immunoassay development.

In this study, multiple anti-TG monoclonal antibodies were screened using indirect ELISA, inhibition ELISA, and Western blot techniques to evaluate their reactivity and specificity. The research also explored the role of antibody orientation and antigen–antibody interactions in successful matched pair formation for sandwich ELISA assays.

By implementing a biotin–streptavidin capture strategy, the study improved antibody orientation and enabled successful identification of functional matched antibody pairs. This approach provides an effective framework for screening antibodies against structurally complex antigens, supporting the development of more sensitive and reliable immunoassays.