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Recombinant CK-MB is produced in E. coli and purified to ≥90% purity. CK-MB is a clinically significant cardiac marker used for myocardial infarction diagnostics and assay calibration. The recombinant His-tagged form is formulated in 20 mM Tris-HCl (pH 8.0 ± 0.2), 150 mM NaCl, 5% glycerol, 1 mM EDTA, and 1 mM DTT, ensuring enzyme stability and consistent activity for immunoassays and enzymatic assays.
| Parameter | Details |
|---|---|
| Source | E. coli (Recombinant expression) |
| Application | Suitable for CK-MB immunoassays, enzymatic activity assays, calibrators, cardiac biomarker diagnostics, and research applications |
| Purity Analysis | SDS-PAGE: Major band at ~90 kDa; CK Isoenzyme Purity: CK-MB 97.8%, CK-MM 2.2%, CK-BB 0% |
| Immunological Activity | CK-MB Immunoassay (Roche Cobas UV): 0.54 mg/mL; Host Cell Protein: Negative (Western Blot) |
| Enzymatic Activity | Total CPK Activity: 746.17 U/mL; Specific Activity (Roche): 1381.79 U/mg |
| Presentation | Liquid (aseptically filtered), 20 mM Tris-HCl pH 8.0 ± 0.2, 150 mM NaCl, 5% glycerol, 1 mM EDTA, 1 mM DTT |
| Storage | −80°C in working aliquots to avoid freeze–thaw cycles |
| Other Information | Batch: ErCKMB0066/25; Cat No.: FrCKMBh-207; Grade: Pure (≥90%) |
| Bioburden | Negative |
| Viral Testing | Not applicable (recombinant E. coli origin) |
| Material Safety Note | Not for human or animal consumption; for in-vitro research use only. Follow biosafety precautions and discard as per regulatory guidelines. |
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