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IgG Fc Immunogen Grade is a highly purified human IgG Fc fragment, suitable for generating antibodies, assay development, immunization, and immunochemical applications. The product is formulated in 50 mM Tris (pH 8.0) with 200 mM sodium chloride and 0.05% sodium azide for stability. Purity exceeds 98% by densitometry, making it ideal for high-sensitivity immunological studies.
| Parameter | Details |
|---|---|
| Source | Human IgG |
| Application | Antibody production, immunization, immunogen preparation, assay development, IEP, Western blot standards |
| Purity Analysis | SDS-PAGE (12%, Non-Reducing): >98% purity; Major band at ~50 kDa |
| Immunological Activity | IEP: Single arc; Western Blot (12% NR): Major band at ~50 kDa |
| Protein Concentration | 20.88 mg/mL (OD280 method) |
| Presentation | Liquid, 50 mM Tris (pH 8.0), 200 mM NaCl, 0.05% sodium azide |
| Storage | −20°C in aliquots to avoid freeze–thaw cycles |
| Other Information | Grade: High Pure |
| Bioburden | ≤10 CFU/mL |
| Viral Testing | CLIA (US-FDA Approved) – Negative for HBsAg, Anti-HCV, Anti-HIV I & II |
| Material Safety Note | Not for human/animal use. For in-vitro research only. Follow biosafety precautions. Dispose as per regulations. |
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