Myoglobin

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Myoglobin

This recombinant human Myoglobin (Holo form, His-tagged) is a highly purified protein preparation suitable for immunization, immunogen preparation, assay development, antibody generation, and immunochemical applications. The product is lyophilized from an aseptically filtered formulation containing 20 mM Tris (pH 8.0 ± 0.3) with 100 mM sodium chloride. Manufactured in E. coli, the protein is supplied at high purity (>95% by SDS-PAGE and SEC-HPLC), making it ideal for sensitive analytical and research applications.

Parameter	Details
Source	E. coli expressed recombinant human Myoglobin (Holo form, His-tagged)
Applications	Immunization, antibody production, immunogen preparation, assay development, Western blot control, ELISA standard, protein-interaction studies
Purity Analysis	SDS-PAGE (15%) CBB stained: >95% purity; Major band at ~17.5 kDa SEC-HPLC: >95% monomeric protein
Identification / Immunological Activity	Western Blot: Positive band at ~17.5 kDa using HRP-conjugated Anti-Myoglobin antibody
Protein Activity	1.03 mg/mL (ECLIA)
Protein Concentration	1.11 mg/mL (Bradford Assay)
Presentation	Lyophilized powder; to reconstitute in 0.5 mL of solution (20% glycerol, 5 mM DTT)
Formulation Buffer	20 mM Tris (pH 8.0 ± 0.3) containing 100 mM NaCl
Storage	−20°C in aliquots; avoid repeated freeze–thaw cycles
Other Information	Grade: Pure (≥90%)
Bioburden	Negative (plate count)
Viral Testing	CLIA (US-FDA Approved): Negative for HBsAg, Anti-HCV, Anti-HIV I & II
Material Safety Note	Not for human/animal consumption. For in-vitro research only. Follow standard biosafety procedures. Dispose as per regulations.

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Myoglobin

Myoglobin

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